The purpose of this investigation is to determine the relationship between in-game workload and injury in National Basketball Association (NBA) athletes. SportVU historical data for the 2013-2017 seasons were analyzed to determine the relationship between in-game workload variables and injury as well as if the selected variables could predict injury. Specifically, the acute, chronic, and acute: chronic workload ratio (ACWR) groupings of less than 0.8, between 0.8 and 1.3, and greaater than 1.3 and Area Under Receiver Operator Curve (AUROC) analysis were performed to determine predictive accuracy. For each of the selected workload variables, results revealed proportions of injury were higher when ACWR exceeded 1.3, while the AUROC resulted in poor prediction ability. These data suggest a relationship exists between in-game workload variables and injuries in the NBA, however this relationship is a poor predictor of injury. The results provide insight into the relationship between in-game workload and injury in NBA athletes and utlimately improves the ability of NBA practitioners to manage athletes away from injury risk.