Functional tests have been primarily used as a measure to assess an athlete’s readiness to return to sport. The purpose of this prospective cohort study was to determine if the following tests; the standing long jump (SLJ), the single leg hop (SLH) for distance, and the lower extremity functional test (LEFT) could be used as a pre-season screening tools to identify collegiate athletes at risk for a time-loss sport-related low back or lower extremity injury. It was expected that athletes with shorter jump and hop distances and slower LEFT scores would be at greater risk for injury. A secondary purpose of this study was to examine relationships between each test and preseason training activities in a collegiate athletic population.