Objective: This investigation aimed to evaluate the effectiveness of a brief mindfulness-based intervention (MBI) on perceived stress and trait mindfulness. Participants were licensed speech-language pathologists (SLPs) who worked full-time in healthcare and reported experiencing moderate levels of stress. Method: This investigation utilized a single-subject, A-B-A experimental design. Three participants completed this virtual, asynchronous investigation. Participants used a commercially available mobile application (app) to complete one short, guided meditation each day for 15 days. Each session was between 6-17 minutes long. The primary dependent variable, perceived stress, was measured after every third mindfulness session, using the 10-item Perceived Stress Scale (PSS-10). The secondary dependent variable, trait mindfulness, was measured before and after the investigation phase using the Mindful Attention and Awareness Scale (MAAS). Results: All three participants demonstrated statistically significant reductions in perceived stress compared to their baseline scores. When comparing pre- and post-test mindfulness scores, two participants demonstrated a positive change in trait mindfulness. Conclusion: A 15-day mindfulness-based intervention was effective in reducing perceived levels of stress in SLPs working in healthcare. These findings illustrate that brief MBIs might be a potential solution to the growing problem of chronic stress experienced by healthcare workers.