Law enforcement officers (LEOs) must adhere to both public and institutional expectations. The evolving duties, stressful environments, and occupational hazards continuously challenge the LEO to correctly perceive and respond to threats in limited time frames. To reduce perceptual error, strategies to improve the officer’s ability to respond are desired. The purpose of Arm 1 was to examine relationships between heart rate variability (HRV) versus tartle response time, and handgun mechanics in a simulated domestic ambush. The purpose of Arm 2 was to examine relationships between physical activity rating and morning HRV.